The Ebley bypass has reopened after flooding caused it to be shut for most of the morning.
Stageocach West reported severe delays to services 61, 64, 66 & 866 as a result.
The nearby River Frome has risen highly as this video from Stroud District Council shows.
Delays are also expected this morning on the A46 at Salmon Springs this morning as highways crews work there.
Other roads believed to have been affected by flooding, or increased traffic as a result of flooding elsewhere, include Selsley Road, Selsley Hill and the A419 between Stroud and the M5.
Traffic was particularly bad on the road from the motorway to Stonehouse and then on to Ebley, although this does appear to be easing.
The road also flooded outside the Old Fleece pub last night.
Heavy rain has left deep standing water outside the pub in North Woodchester last night.
Cars have broken down while attempting to drive along the road outside.
Pub owner Nick Beardsley said: "Whilst you need a boat to get to the Old Fleece it hasn't stopped a full house this evening. Police have just arrived to assist the many cars broken down in the deep waters."
There are also reports of lorries stuck in Woodchester.
Gloucestershire police say they have taken a lot of calls about flooding across the county.
A Tweet from their force control room stated: "Please DO be considerate of other drivers and pedestrians.
"DO NOT drive into large puddles."
The M5 Northbound entry slip road at J11A has now reopened, as has the A38 at Berkeley.
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